Wahyu Widowati, Dani Dani, Vera Vera, Teresa L. Wargasetia, Fanny Rahardja, Fen Tih, Philips Onggowidjaja, Rita Tjokropranoto, Fadhilah H. Zahiroh, Rizal Azis, Didik Priyandoko, Wahyu Surakusumah, Dhanar S. Hadiprasetyo
Objectives: Ultraviolet (UV) exposure can hasten the aging process of the skin. The use of chemicals for anti-aging has long-term adverse effects. The natural ingredients of snake fruit (Salacca zalacca L.) are known to have bioactive properties such as polyphenols, flavonoids, chlorogenic acid, and caffeic acid which have antiaging potential. The study aims to ascertain the potential of S. zalacca Extract (SZE) as an antiaging agent by in vitro assay. Methods: The SZE compound content was analyzed by LC-MS/MS. SZE viability test on human skin fibroblast (BJ) cells was carried out using the WST assay. BJ cells were UV-induced as a cell model of premature aging. SZE 6.25, 12.5, and 25 μg/mL were administered to UV-induced BJ cells. The gene expression of COL1A1, MMP-1, FGF-2, and GPX-1 were analyzed by quantitative Real-Time PCR. Elastin (ELN), Hyaluronidase (HAse), Cyclooxigenase-2 (COX-2), 8-Hydroxydeoxyguanosine (8-OHdG), and Melatonin (MT) protein levels were analyzed by ELISA assay. The apoptosis of BJ cells was analyzed using flow cytometry. One-way ANOVA in SPSS Software was used for statistical analysis. Results: Treatment with SZE increased COL1A1, FGF-2, and GPX-1 gene expression and also decreased MMP-1 gene expression. SZE also increased ELN and MT levels in UV-induced BJ cells. After SZE treatment, the protein levels of HAse, COX-2, and 8-OHdG decreased compared to the positive control. SZE also succeeded in maintaining the lives of BJ cells and reducing apoptosis in BJ cells. Conclusions: SZE has the potential to be an antiaging agent by in vitro assay. © 2025 The Authors
Faculty of Medicine, Maranatha Christian University, West Java, Bandung, Indonesia; Bimolecular and Biomedical Research Center, Aretha Medika Utama, West Java, Bandung, Indonesia; Biomedical Engineering, Department of Electrical Engineering, Faculty of Engineering, Universitas Indonesia, West Java, Depok, Indonesia; Biology Study Program, Faculty of Mathematics and Science Education, Universitas Pendidikan Indonesia, West Java, Bandung, Indonesia; Faculty of Pharmacy, Universitas Jenderal Achmad Yani, West Java, Cimahi, Indonesia
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