Syifa F. Syihab, Akhmaloka, Fida Madayanti Warganegara, Rina Budi Satiyarti
Lipase is a hydrolytic enzyme that has been used widely in numerous industries, such as detergent, food, cosmetics, and pharmacy. The enzyme can catalyze esterification, interesterification, and transesterification reactions in a nonaqueous environment. Lipase could catalyze the hydrolysis reaction of fatty acids. Therefore, it can be used to produce medium-chain fatty acids from vegetable oils. The composting process occurs at various temperatures, resulting in a unique environment that can be used to obtain thermophilic microorganisms that produce enzymes. Lipase-producing bacteria from domestic compost were successfully cultivated in the culture broth and identified as Brevibacillus sp. The objective of this research is to purify and characterize enzymes isolated from Brevibacillus bacteria. The microbes were isolated from domestic waste compost at the peak of the thermogenic phase. Extracellular lipase was produced in nutrient broth and purified using acetone precipitation and anion exchange chromatography on a DEAE-Sepharose Fast Flow matrix. An extracellular lipase was purified 5.1 folds with an overall yield of 15%. Further purification of the enzyme using ion-exchange chromatography revealed that the enzyme had a molecular weight of 55 kDa. Characterization of optimum pH, temperature, and substrate specificity was carried out against the purified lipase. The optimum activity of the enzyme was at 65-70°C and pH 9, suggesting that it is a thermostable and alkali-tolerant lipase. The enzyme showed the highest activity towards p-nitrophenyl decanoate and laurate, respectively. Protein purification using acetone precipitation and anion exchange chromatography produces lipases that are resistant to high temperatures and are active in alkaline pH. Lipase produced from Brevibacillus sp. also has specificity towards medium-chain fatty acids, thus it can be used in industries that use medium-chain fatty acids. © 2024 Author(s).
Nutrition Study Program, Faculty of Sports Education and Health, Universitas Pendidikan Indonesia, Bandung, 40154, Indonesia; Chemistry Study Program, Faculty of Science and Computer, Universitas Pertamina, Jakarta, 12220, Indonesia; Chemistry Department, Faculty of Mathematics and Natural Science, Institut Teknologi Bandung, Bandung, 40132, Indonesia; Biology Education Study Program, Faculty of Tarbiyah and Teacher Training, Universitas Islam Negeri Raden Intan Lampung, Lampung, 35131, Indonesia
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