Jia Wang, Huayue Zhang, Ashish Kaul, Kejuan Li, Didik Priyandoko, Sunil C. Kaul, Renu Wadhwa
Withania somnifera (Ashwagandha) is used in Indian traditional medicine, Ayurveda, and is believed to have a variety of health-promoting effects. The molecular mechanisms and pathways underlying these effects have not yet been sufficiently explored. In this study, we investigated the effect of Ashwagandha extracts and their major withanolides (withaferin A and withanone) on muscle cell differentiation using C2C12 myoblasts. We found that withaferin A and withanone and Ashwagandha extracts possessing different ratios of these active ingredients have different effects on the differentiation of C2C12. Withanone and withanone-rich extracts caused stronger differentiation of myoblasts to myotubes, deaggregation of heat-and metal-stress-induced aggregated proteins, and activation of hypoxia and autophagy pathways. Of note, the Parkinson’s disease model of Drosophila that possess a neuromuscular disorder showed improvement in their flight and climbing activity, suggesting the potential of Ashwagandha withanolides for the management of muscle repair and activity. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
AIST-INDIA DAILAB, DBT-AIST International Center for Translational & Environm ental Research (DAICENTER), National Institute of Advanced Industrial Science & Technology (AIST), Tsukuba, 3058565, Japan; College of Life Science, Sichuan Normal University, Chengdu, 610066, China; Department of Biology, Universitas Pendidikan Indonesia, Bangdung, 40154, Indonesia
Research at a Glance
Register to unlock
Topics & SDG Alignment
Register to unlock
Collaboration
Register to unlock
Author Profile (Selected)
Register to unlock
References Overview
Register to unlock
Journal & Source
Register to unlock
Metadata & Integrity
Register to unlock