Atqiya Muslihati, Chandra Wulandari, Ni Luh Wulan Septiani, Gilang Gumilar, Agus Subagio, Ida Hamidah, Nugraha Nugraha, Erwin Peiner, Hutomo Suryo Wasisto, Brian Yuliarto
The detection of Escherichia coli (E. coli) in food and water is critical for public health, yet existing methods often lack sensitivity, speed, or field applicability. Here, we develop an electrochemical biosensor based on Mn-doped Co zeolitic imidazolate framework (ZIF-67) functionalized with anti-O antibody for sensitive E. coli detection. Mn incorporation induces phase reconstruction, surface area enhancement, and electron transfer. Antibody conjugation modulates wettability, introduces amide I and II vibrational modes, and selectively blocks electron transfer upon bacterial binding. The biosensor exhibits a linear range of 10 to 1010 CFU mL–1 with a 1 CFU mL–1 detection limit, outperforming optical and other metal organic framework-based sensors. It can discriminate non-target bacteria (Salmonella, Pseudomonas aeruginosa, Staphylococcus aureus), maintain >80% sensitivity over 5 weeks, and recover 93.10 –107.52% E. coli spiked in tap water. This work suggests the tremendous potential for on-site pathogen monitoring. © The Author(s) 2025.
Doctoral Program of Engineering Physics, Faculty of Industrial Technology, Institut Teknologi Bandung, Bandung, Indonesia; Advanced Functional Material Laboratory, Faculty of Industrial Technology, Institut Teknologi Bandung, Bandung, Indonesia; PT Biostark Analitika Inovasi, Bandung, Indonesia; Research Center for Electronics, National Research and Innovation Agency (BRIN), Bandung, Indonesia; Department of Physics, Faculty of Science and Mathematics, Universitas Diponegoro, Semarang, Indonesia; Department of Engineering and Vocational Education, Universitas Pendidikan Indonesia, Bandung, Indonesia; Research Center for Nanosciences and Nanotechnology (RCNN), Institut Teknologi Bandung, Bandung, Indonesia; Institute of Semiconductor Technology (IHT), Laboratory for Emerging Nanometrology (LENA), Technische Universität Braunschweig, Braunschweig, Germany
Research at a Glance
Register to unlock
Topics & SDG Alignment
Register to unlock
Collaboration
Register to unlock
Author Profile (Selected)
Register to unlock
References Overview
Register to unlock
Journal & Source
Register to unlock
Metadata & Integrity
Register to unlock