Wahyu Widowati, Ahmad Faried, Diki Diki, Deni Rahmat, Annisa Firdaus Sutendi, Hanna Sari Widya Kusuma, Nindia Salsabila Mia Dewi, Fadhilah Haifa Zahiroh, Didik Priyandoko, Wahyu Surakusumah, Rizal Azis, Dhanar Septyawan Hadiprasetyo, Rita Tjokropranoto, Philips Onggowidjaja
Glioma is a type of brain tumor that start from neuroglial stem cells. Despite advancements in surgery and additional adjuvant therapy such as Temozolomide (TMZ), treating this tumor continues to present a challenge with notable side effects, such as toxicity and resistance to treatment. Therefore, the investigation of natural remedies becomes intriguing. This study examines the potential of nano-particle-formulated curcumin, a compound derived from Curcuma longa L as a promising antitumor agent. The research was carried out in vitro by treating Glioblastoma multiforme (GBM) cell with various concentrations (25, 50, and 100 µg/mL) of nano-particle of curcumin (NC) and TMZ 300 µM. qRT-PCR was employed to assess the relative expression of mRNA Caspase 3 (Casp-3), Insulin-like Growth Factor Binding Protein 2 (IGFBP-2), Epidermal Growth Factor Receptor (EGFR), and Extracellular Signal-Regulated Kinases (ERK). While the proportion of live, necrotic and apoptotic cells was employed utilizing flow cytometry. GBM shows a high expression of growth factors and a low expression of apoptotic gene. The treatment using NC reduced the expression of IGFBP-2, EGFR, and ERK genes, while increasing Casp-3. GBM cells shows higher apoptotic activities and lower necrotic activities after the addition of NC. In comparison to TMZ, NC demonstrates a promise as an anti-tumor agent, particularly for brain tumors, with the optimal dosage identified to be 25 µg/mL. © 2025 Marmara University Press.
Faculty of Medicine, Maranatha Chirstian University, Bandung, Indonesia; Department of Neurosurgery, Faculty of Medicine, Padjadjaran University, Bandung, Indonesia; Department of Biology, Faculty of Science and Technology, Universitas Terbuka, Tangerang Selatan, Indonesia; Faculty of Pharmacy, Universitas Pancasila, Jakarta, Indonesia; Biomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung, Indonesia; Biology Study Program, Faculty of Mathematics and Natural Science Education, Universitas Pendidikan Indonesia, Bandung, Indonesia; Biomedical Engineering, Department of Electrical Engineering, Faculty of Engineering, Universitas Indonesia, Depok, Indonesia; Faculty of Pharmacy, Jenderal Achmad Yani University, Cimahi, Indonesia
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