Hamidie Ronald Daniel Ray, Cardial L. O. Leo Penu, Ambar Susanti, Kazumi Masuda
This study has been made to determine the effect of dorsomorphin as one of the reagents on adenosine monophosphate-activated protein kinase (AMPK) inhibitor in skeletal muscle. In the experimental procedure, four and eight mg/kg-body weight/day of dorsomorphin were injected every two days for 12 consecutive days (6 times of injection) to Wistar rat. Gastrocnemius muscle immediately harvested from the Wistar rat, continued by protein expression analysis. The analysis also involved regulated mitochondrial biogenesis, including phosphorylation AMPK, SIRT, PGC-1α, VDAC and COX-IV. Results indicated that 8 mg/kg – body weight/day of dorsomorphin decreased protein expression. In the low dosage, dose, such as 4 mg/kg-body weight/day of dorsomorphin, had no impact on decreasing protein expression. Dorsomorphin decreases phosphorylation of AMPK and mitochondrial biomarker, including marker included VDAC and COX-IV on rats skeletal muscle through AMPK-SIRT-PGC-1α pathway. © School of Engineering, Taylor’s University.
Departemen Ilmu Keolahragaan, Universitas Pendidikan Indonesia, Jl. Dr. Setiabudi no 229, Bandung, 40154, Indonesia; Kanazawa University, Kakuma-machi, Kanazawa, 920-1192, Japan; Jurusan Peternakan, Politeknik Pertanian Negeri Kupang, Jl. Prof. Herman Yohanes Lasiyana, Kupang, 85011, Indonesia; Universitas KH.A.Wahab Hasbullah, Jl.Garuda No 9, Jombang, 61451, Indonesia
Research at a Glance
Register to unlock
Topics & SDG Alignment
Register to unlock
Collaboration
Register to unlock
Author Profile (Selected)
Register to unlock
References Overview
Register to unlock
Journal & Source
Register to unlock
Metadata & Integrity
Register to unlock