Y. Sanjaya, V.R. Ocampo, B.L. Caoili
The characteristics of six Beauveria bassiana isolates against Tetranychus kanzawai were investigated. The investigated aspects were B. bassiana isolates': (1) pathogenicity to T. kanzawai, (2) cellular characteristics, (3) infection process (4) phylogeny. The isolates were planted onto potato dextrose agar (PDA) in flat bottle flasks and incubated at certain room condition, i.e. 26±1°C temperature and 12-hour photoperiod, for a maximum of 21 days. The results showed that isolates' phatogenicity level decreased in the following order: Bb6>Bb5>Bb4>Bb3>Bb1>Bb2. The surface of Bb4 and Bb5 isolates were yellowish-white, while Bb6 had a white color. The bottom of Bb4 had brown and white colors while both Bb5 and Bb6, on the other hand, had yellow color. All isolates had round shapes. Bb6 had the fastest sporulation growth of 3 days post inoculation while others grew approximately at 4 days post inoculation. The ITS region examination showed that three B. bassiana isolates were related, in which Bb4 and Bb5 (from Indonesia) were closely related and Bb6 (from Philippines) was distantly related.
Indonesia University of Education, Jl Setiabudi No. 229, Bandung, 40154, Indonesia; Crop Protection Cluster, College of Agriculture, University Philippines Los Baños College, Laguna, 4031, Philippines
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